{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"0d951448-423b-46c7-aa0e-7e65c0e247d3","name":"data-development-vitro-method-propagation-basin-big-sagebrush-artemisia-tridentata-subsp","title":"Data from: Development of an in vitro method of propagation for basin big sagebrush (Artemisia tridentata subsp. tridentata)","author":"Rachael Barron, Peggy Martinez, Marcelo Serpe, Sven Buerki","author_email":"svenbuerki@boisestate.edu","maintainer":"RCDS Data Repository","maintainer_email":"rcds-web@uidaho.edu","license_title":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/","notes":"\u003Cp\u003EData and code files included within release v1.0 from the \u003Ca href=\u0022https:\/\/github.com\/svenbuerki\/Sagebrush_rooting_in_vitro_prop\u0022\u003ESagebrush_in_vitro_prop GitRepo\u003C\/a\u003E. Please visit the associated \u003Ca href=\u0022https:\/\/svenbuerki.github.io\/Sagebrush_rooting_in_vitro_prop\/index.html\u0022\u003Eproject website\u003C\/a\u003E for specific details about project protocols and workflow.\u003C\/p\u003E\n\u003Cp\u003EBasin big sagebrush (\u003Cem\u003EArtemisia tridentata\u003C\/em\u003E subsp. \u003Cem\u003Etridentata\u003C\/em\u003E) is a keystone species of the sagebrush steppe, a widespread ecosystem of western North America threatened by climate change. The study\u2019s goal was to develop an in vitro method of propagation for this taxon to support genome sequencing and genotype-by-environment research on drought tolerance. Such research may ultimately facilitate the reintroduction of big sagebrush in degraded habitats. Seedlings were generated from two diploid mother plants (2n = 2x = 18) collected in environments with contrasting precipitation regimes. The effects of IBA and NAA on rooting of shoot tips were tested on 45 individuals and 15 shoot tips per individual. Growth regulator and individual-seedling effects on percent rooting and roots per shoot tip were evaluated using statistical and clustering analyses. Furthermore, rooted shoot tips were transferred into new media to ascertain their continued growth in vitro. The results suggest that \u003Cem\u003EA. tridentata\u003C\/em\u003E is an outbred species, as shown by individuals\u2019 effect on rooting and growth. IBA addition was the most effective method for promoting adventitious rooting, especially in top-performing individuals. These individuals also have high survival and growth rates upon transferring to new media, making them suitable candidates for generating biomass for genome sequencing and producing clones for genotype-by-environment research.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EData Use\u003C\/strong\u003E\u003Cbr \/\u003E\n\u003Cem\u003ELicense\u003C\/em\u003E: \u003Ca href=\u0022https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/\u0022\u003ECC-BY-NC-SA 4.0\u003C\/a\u003E\u003Cbr \/\u003E\n\u003Cem\u003ERecommended Citation\u003C\/em\u003E: Barron, R., Martinez, P., Serpe, M., \u0026amp; Buerki, S. (2021). Data from: Development of an in vitro method of propagation for basin big sagebrush (Artemisia tridentata subsp. tridentata) [Data set]. University of Idaho. \u003Ca href=\u0022https:\/\/doi.org\/10.7923\/E3V2-SK66\u0022\u003Ehttps:\/\/doi.org\/10.7923\/E3V2-SK66\u003C\/a\u003E\u003C\/p\u003E\n","url":"https:\/\/dkan.nkn.uidaho.edu\/dataset\/data-development-vitro-method-propagation-basin-big-sagebrush-artemisia-tridentata-subsp","state":"Active","log_message":"Update to resource Data Access v1.0 | Data from: Development of an in vitro method of propagation for basin big sagebrush (Artemisia tridentata subsp. tridentata)","private":true,"revision_timestamp":"Thu, 10\/06\/2022 - 10:43","metadata_created":"Wed, 02\/17\/2021 - 10:52","metadata_modified":"Thu, 10\/06\/2022 - 10:43","creator_user_id":"6cc16f2c-77c2-4d12-ac06-56bbb86b535b","type":"Dataset","resources":[{"id":"1157e534-cd0c-488d-adc6-1e1aef36a534","revision_id":"","url":"https:\/\/dkan.nkn.uidaho.edu\/sites\/default\/files\/Sagebrush_rooting_in_vitro_prop-1.0.zip","description":"","format":"zip","state":"Active","revision_timestamp":"Thu, 10\/06\/2022 - 10:43","name":"Data Access v1.0 | Data from: Development of an in vitro method of propagation for basin big sagebrush (Artemisia tridentata subsp. tridentata)","mimetype":"application\/zip","size":"21.23 MB","created":"Wed, 02\/09\/2022 - 12:00","resource_group_id":"1812a312-11a4-492d-960b-9a78b4abfe0c","last_modified":"Date changed  Thu, 10\/06\/2022 - 10:43"},{"id":"05bb6932-e69f-4706-a7dd-c5c86d0aea8b","revision_id":"","url":"https:\/\/dkan.nkn.uidaho.edu\/sites\/default\/files\/e3v2-sk66.xml","description":"","format":"xml","state":"Active","revision_timestamp":"Thu, 10\/06\/2022 - 10:43","name":"Metadata Access | Data from: Development of an in vitro method of propagation for basin big sagebrush (Artemisia tridentata subsp. tridentata)","mimetype":"text\/xml","size":"5.3 KB","created":"Thu, 10\/06\/2022 - 10:43","resource_group_id":"1812a312-11a4-492d-960b-9a78b4abfe0c","last_modified":"Date changed  Thu, 10\/06\/2022 - 10:43"}],"tags":[{"id":"d7f46c33-19b1-4acb-9a17-c37700ec2e04","vocabulary_id":"2","name":"drought"},{"id":"604e1913-850d-4ff3-ac2f-bf17c6c6be32","vocabulary_id":"2","name":"in vitro culture"},{"id":"e0e801c8-e070-408e-b5ea-bc7776de05d1","vocabulary_id":"2","name":"genotype"},{"id":"cb317fbe-fb50-4a46-930e-32a2db0a9214","vocabulary_id":"2","name":"sagebrush"},{"id":"64fe7ed2-4e6f-4da1-a120-be0ad6275832","vocabulary_id":"2","name":"plant growth regulators"}],"groups":[{"description":"\u003Cp\u003EGEM3 is an NSF EPSCoR research program seeking to understand how genetic diversity and phenotypic plasticity affect species response to environmental change, shaping both population response and adaptive capacity.\u003C\/p\u003E\n\u003Cp\u003EVisit them at: \u003Ca href=\u0022https:\/\/www.idahogem3.org\u0022\u003Ehttps:\/\/www.idahogem3.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"1812a312-11a4-492d-960b-9a78b4abfe0c","image_display_url":"https:\/\/dkan.nkn.uidaho.edu\/sites\/default\/files\/GEM3_nov5_logo.png","title":"EPSCoR GEM3","name":"group\/epscor-gem3"},{"description":"\u003Cp\u003EBoise State University is a Carnegie-classified doctoral research university and our students have opportunities to work with talented and accomplished faculty on research, even as undergraduates.  Our students go into the workforce better prepared, with expertise outside of their major by taking advantage of opportunities such as certificates in business anthropology, entrepreneurship, or cybersecurity.  \u003C\/p\u003E\n\u003Cp\u003ELearn more at \u003Ca href=\u0022https:\/\/www.boisestate.edu\/\u0022\u003Ehttps:\/\/www.boisestate.edu\/\u003C\/a\u003E\u003C\/p\u003E\n","id":"bff301ea-b742-427b-954e-e6a326c2ea45","image_display_url":"https:\/\/dkan.nkn.uidaho.edu\/sites\/default\/files\/bsu_logo.jpg","title":"Boise State University","name":"group\/boise-state-university"},{"description":"\u003Cp\u003EThe primary objective of Idaho EPSCoR is to stimulate research in niche areas that can become fully competitive in the disciplinary and multidisciplinary research programs of the National Science Foundation and other relevant agencies. Idaho EPSCoR provides support for sustainable increases in Research and Development capacity and advances science and engineering capabilities within the state. \u003C\/p\u003E\n\u003Cp\u003EVisit them at \u003Ca href=\u0022https:\/\/www.idahoepscor.org\u0022\u003Ehttps:\/\/www.idahoepscor.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"e696b239-9ecb-412e-b032-03a75b2b9fd6","image_display_url":"https:\/\/dkan.nkn.uidaho.edu\/sites\/default\/files\/Idaho_epscor_logo_no_white_background.png","title":"Idaho EPSCoR","name":"group\/idaho-epscor"}]}]}<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN"
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